Figure 5.

The pH_i-buffering capacities of Mi.III⁺ erythrocytes were superior. Fresh RBCs were loaded with fluorescent pH indicator SNARF-1, and its intracellular pH measurement at pH_out 7.5 was measured by flow cytometry. In the absence of extracellular bicarbonate, the control cells became more acidified than Mi.III. Depletion of extracellular Cl⁻ maximized HCO₃⁻ loading for both Mi.III⁺ and the control cells, and diminished their pH_i differences. The number of donors tested was indicated next to each bar. Data are expressed as mean ± SE; *P < .05.