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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Cell Motil Cytoskeleton. 2009 Aug;66(8):524–534. doi: 10.1002/cm.20356

Figure 1.

Figure 1

Characterization of fascin1-deficient mice. a. Fascin1 gene disruption by a retrovirus insertion. An ES clone (OST124903) has a retrovirus insertion at the nucleotide 1,726 between exon1 and exon2, resulting in the disruption of fascin1 gene. b. The nucleotide number 1 is the translational start site of the mouse fascin1 gene (MGI:1352745). Western blot analysis to confirm that fascin1 KO mice do not express fascin1. Lane 1, Brain from wild-type mice; lane 2, Molecular weight markers; lanes 3-7 are from fascin1 KO mice (3, brain; 4, liver, 5, embryonic brain; 6 placenta; 7, spleen). c, RT-PCR analysis of wild type (+/+) and fascin1 KO (-/-) mouse tissues (lanes 1-6, brain; lanes 7-12, retina; lanes 13-18, testis; lanes 19-24, embryonic brain). Three different primer sets were used to detect fascin1 (labeled “b”), fascin2 (labeled “r”) and fascin3 (labeled “t”) mRNA. An actin primer set was used as a control. Note that both adult and embryonic brain tissues from fasin1-deficient mice did not express fascin2 or fascin3 paralogues.