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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Prostaglandins Other Lipid Mediat. 2009 Mar 25;89(1-2):26–33. doi: 10.1016/j.prostaglandins.2009.03.001

Fig. 6. Effect of Epac-specific and PKA-specific cAMP analog on activation of Rap1 and CREB in RSF.

Fig. 6

A, Serum-starved RSF were stimulated with 8-CPT-2-O-Me-cAMP (Epac-specific cAMP analog, 500 μM) and 6-Bnz-cAMP (PKA-specific cAMP analog, 500 μM) for 45 min. Lysates from the cells were subjected to Rap1 pull down assay. B, Serum-starved RSF were stimulated with 8-CPT-2-O-Me-cAMP (Epac-specific cAMP analog, 500 μM) and 6-Bnz-cAMP (PKA -specific cAMP analog, 500 μM) for 10 min. Levels of phospho-CREB and total CREB were analyzed by Western blotting. Blots are representative data and values are expressed as means ± standard errors from the indicated number of patients with RA in each panel.