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High doses of Shaker B induce three distinct levels of subconductance. (A) RyR2 recordings of a Ca2+/caffeine-activated channel at Vm = +10 mV in the presence of 20 µM Shaker B. s1, s2 and s3 indicate the conductance of the different substates. (B) Amplitude histograms and fits of RyR2 channels exposed to 20 µM Shaker B.
