Table 1.
Primers for RT-PCR amplification of growth factors, transcription factors, extracellular matrix genes, and house keeping genes used to characterized dental follicle derived and alveolar bone derived cell lines.
| Gene | Orientation | Sequence |
|---|---|---|
| SV40 T antigen | S | GACTTTGGAGGCTTCTGGATGCAACTGAG |
| SV40 T antigen | AS | AGCAGACACTCTATGCCTGTGTGGAGTAAG |
| Amelogenin | S | AACCATCAAGAAATGGGG |
| Amelogenin | AS | TCGGTTCTCTCATTTTCTG |
| DSPP | S | ACGGGATAGAGGAGGATGA |
| DSPP | AS | TTCCACTGAGCCTTCCCAGA |
| Osteocalcin | S | CTTGCCTTCTGCCTGGGTGTCC |
| Osteocalcin | AS | GATGCGTTTGTAGGCGGTCTTCA |
| Osteopontin | S | GACCATGAGATTGGCAGTGATTTG |
| Osteopontin | AS | TGATGTTCCAGGCTGGCTTTG |
| Osteonectin (Sparc) | S | GTCCCACACTGAGCTGGC |
| Osteonectin (Sparc) | AS | AAGCACAGAGTCTGGGTGAGT |
| Tropoelastin | S | CTTGGTGGAGCCGGAGGATTG |
| Tropoelastin | AS | GGAGGGGGTGGTCATAGAAGGTTT |
| β-actin | S | GAGCAAGAGAGGTATCCTGAC |
| β-actin | AS | TTCATGGATGCCACAGGATTC |
| FGF 9 | S | GACTCGTTCTTGTGCTTAAAAGCCGAGTC |
| FGF 9 | AS | GCGTCCTGCACACCGAAATA |
| BMP 4 | S | CACAGTAGCCGAGCCAACACTGTG |
| BMP 4 | AS | TGGTCCCTGGGATGTTCTCC |
| Runx 2 | S | CACAGGCGACAGTCCCAACTTCCTGG |
| Runx 2 | AS | CTCTCCGAGGGCTACAACCTT |
| Type I Collagen | S | GAACCAGCCGATGATGCTAACGTGGTC |
| Type I Collagen | AS | GGCTGCGGATGTTCTCAATCT |