Table 1.
Kinetic parameters of LSD1 with the H3K4 21-mer dimethylated peptide as a substrate*
| Kinetic parameter | Value |
|---|---|
| kcat/K21-mer (mM−1 s−1) | 38 ± 2a |
| kcat/KO2 (mM−1 s−1) | 1.0 ± 0.2b |
| K21-mer (μM) | 2.6 ± 0.2a |
| KO2 (μM) | 195 ± 40b |
| kcat (s−1) | 0.199 ± 0.013c |
| kred (s−1) | 0.231 ± 0.004e |
| D(kcat/K21-mer) | 3.2 ± 0.1a,d |
| Dkcat | 2.9 ± 0.2c |
| 3.2 ± 0.1a,d | |
| Dkred | 3.3 ± 0.1e |
*
Conditions: pH 7.5, 25 °C.
a
Determined in air-saturated buffer.
b
Determined using 50 μM H3K4 21-mer dimethylated peptide.
c
Determined using 50 μM H3K4 21-mer dimethylated peptide and 1.1 mM oxygen.
d
Calculated using eq 2.
e
Determined using 200 μM 21-mer dimethylated peptide.