Semiquantitative RT-PCR analysis. RNA from paired myometrium/leiomyoma from 5 different patients was analyzed using specific primer pairs, as indicated and described in Materials and Methods and electrophoresed on agarose gels. Bands were quantitated by scanning densitometry and expressed as relative intensity normalized to GAPDH. B2, B3, B4, B9, B11 – patient designations. N1-5 normal myometrium; L1-5 corresponding leiomyoma samples.