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. Author manuscript; available in PMC: 2010 Mar 15.
Published in final edited form as: J Immunol. 2009 Aug 19;183(6):3906–3914. doi: 10.4049/jimmunol.0900577

Figure 2.

Figure 2

Natural Killer Cells provide an additonal source of IL-13 during delayed expulsion of T. muris. A) The total lymphocyte number of mesenteric lymph nodes and the relative numbers of CD4+ T cells and DX5+ NK cells were calculated after normalising cell percentages derived via flow cytometry to total cell counts. Cell numbers of total cells and respective subpopulations of naive and pooled (n=5) T. muris infected lymph nodes 14, 18 and 26 days p.i. from male and female WT or IL-4KO BALB/c mice are indicated B) The relative expression of IL-13 mRNA transcripts in the pooled MLNs was determined ex vivo via real time PCR using the 2-ΔC(t) method. IL-13 expression was determined relative to the housekeeping gene HPRT and to that of naive mice of the same sex. C) IL-13 expression was determined in UEA-1+ epithelial cells and CD45+ lymphocyte populations isolated from the pooled caecums of infected mice at day 26 p.i. via EDTA/DTT treatment and Percoll gradient centrifugation. Data is representative of two independent experiments.