Skip to main content
. 2009 Jul 16;28(17):2636–2649. doi: 10.1038/emboj.2009.204

Figure 4.

Figure 4

Whole-cell voltage-clamp analysis of muscle cells reveals altered nAChR function in nra-2 and nra-4 mutants. (A) Representative traces for levamisole- (top), nicotine- (middle) and ACh-evoked (bottom) muscle currents in wild-type animals and various mutants of nra-2, nra-4, L- and N-AChR subunits. (B) Normalized mean peak values of levamisole-, nicotine- and ACh-mediated muscle currents in wild-type animals and various nra-2 and nra-4 mutants, and nra-2(ok1731) animals rescued in muscle by NRA-2∷GFP expression. Only GFP-positive cells were patched. (C) Representative traces (left) and mean peak values (right) of GABA-mediated muscle currents were not altered in nra-2(tm1453) mutants, compared with wild type. (D) Normalized mean peak values of levamisole-, nicotine- and ACh-mediated muscle currents in wild-type animals, nra-2(tm1453 or ok1731) mutants as well as in mutants lacking the N-AChR (acr-16(ok789); left) or L-AChR (unc-38(x20); right), and respective double mutants. Displayed are means±s.e.m., statistically significant differences to the wild type are indicated (*P<0.05; **P<0.01; ***P<0.001), as well as the number of animals.