Figure 6.

Single-channel properties of the L-AChR in cell-attached patches of cultured embryonic muscle cells are altered in nra-2(ok1731) mutants. (A) Single-channel currents recorded from wild type and nra-2(ok1731) muscle cells in the presence of 1 μM (upper panel) and 50 μM (lower panel) ACh. Shown are representative traces (left) and open and closed time histograms (right). (B) Single-channel currents activated by 0.1 μM (upper panel) and 50 μM (lower panel) levamisole; representative traces (left) and open time histograms (right). (C) Frequency of channel openings in mutant and wild-type animals. Channel events were counted within the first minute of recording and plotted as events/s. Holding potential in all recordings was −100 mV. Displayed are means±s.d.