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. 2009 Jul 23;28(17):2601–2615. doi: 10.1038/emboj.2009.206

Figure 7.

Figure 7

PARP1 exerts an effect in the same pathway as Mre11 to restart stalled replication forks. DNA fibre analysis of replication fork restart in U2OS cells depleted of PARP1, Mre11 or co-depleted of PARP1 and Mre11. Cells were labelled to analyse fork restart after 2 h HU as in Figure 5. (A) Western blot analysis of PARP1, Mre11 and β-actin in siRNA-treated U2OS cells. (B) Representative images of DNA fibre tracks. (C) Fork restart in PARP1- and/or Mre11-depleted cells. (D) Speed of restarting forks in PARP1- and/or Mre11-depleted cells. IdU fork speeds are shown as percentage of CldU fork speeds. Means and s.d. of three independent experiments are shown. Values marked with asterisks are significantly different from control (*P<0.05 or **P<0.01).