Figure 4.

Enrichment for histone modifications in Spo11^−/− mice. Bound fractions, normalized to Psmb9–STS 1 (the 5′ most flanking STS), of chromatin of whole testes cells from R209xB10 Spo11^+/− (red) and R209xB10 Spo11^−/− (green) pre-puberal littermates are shown for nine sequence tagged sites along the Psmb9 hotspot region. Mann–Whitney statistical test was used to determine the statistical significance of the difference between bound fractions of Spo11^+/− and Spo11^−/− mice for STSs included within the CO hotspot region (STSs 6, 7, 8, 11 and 13). A full-colour version of this figure is available at The EMBO Journal Online.