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. 2009 Jul 30;28(17):2616–2624. doi: 10.1038/emboj.2009.207

Figure 6.

Figure 6

Absence of detectable transcription activity at the Psmb9 hotspot. Northern blot of total RNA (18 μg per lane) prepared from elutriated testes cells of RJ2 and of B10. RJ2 has a shorter wm7 fragment as compared with RB2 but confers the same CO activity at Psmb9 and Hlx1 (Grey et al, 2009). The probes used were PCR products from Psmb9 6F-13R, which includes the hotspot centre, and from Psmb9 15F-18R, which lies outside the hotspot region and includes the most 3′ exon (ex) of the Psmb9 gene. A full-colour version of this figure is available at The EMBO Journal Online.