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Rate constants for the association (kobs) and dissociation (koff) of mant-nucleotides with FeoB1−270
| Protein | Concentration (μM) | mGMPPNP kobs s−1 (20°C) | mGMPPNP koff s−1 (20°C) | mGDP koff,GDP s−1 (20°C) |
|---|---|---|---|---|
| FeoB1−270 | 2.5 | 1.63 | ||
| 5 | 1.93 | 1.85 | 145 (±9) | |
| 10 | 2.68 | |||
| 20 | 4.01 | |||
| 40 | 6.71 | |||
| S150A | 5 | 3.87 | 1.77 | 10 (±0.1) |
| R154A | 5 | 2.14 | 1.89 | 19 (±0.5) |
