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. Author manuscript; available in PMC: 2009 Sep 4.
Published in final edited form as: Analyst. 2008 Jun 5;133(8):975–983. doi: 10.1039/b717527b

Figure 3.

Figure 3

(a) Schematic showing enzymatic amplification of biomarker detection signals via RCA. First, target proteins are labeled with biotin in serum solution and incubated on the antibody array. Primer DNA-conjugated antibodies (i.e. anti-biotin) are then bound to the surface-immobilized biotinylated protein target. Following the binding of circular DNA, a polymerase reaction significantly extends the primer sequence using the circle as a template. This enables the hybridization of multiple labeled oligonucleotides resulting in an amplified fluorescent signal. Adapted from ref. 85. (b) Fluorescence image of an antibody microarray analyzing sera from lung cancer and healthy samples using two-color RCA. Adapted from ref. 29.