Table 1.
Primers used in RT-PCRs.
| Name of gene | Sequence of forward primer | Sequence of reverse primer | Tm | PCR cycle number | Size of product (bp) |
|---|---|---|---|---|---|
| Emx1 | GAGAAGAACCACTACGTGGTGGG | GCCCGTGTCATTAAGAGAGAGAC | 62°C | 40 | 641 |
| GAPDH | GAAGGTGAAGGTCGGAGTC | GAAGATGGTGATGGGATTTC | 52°C | 21 | 226 |
| Gli1 | TCTCAAACTGCCCAGCTTGTC | AGGCTCTTGAACCTCTGGACTC | 58°C | 40 | 432 |
| Gli2 | GACACATCAAAGAGCAAGGATTG | ACCGTGGACAGAATGAGGCT | 55°C | 40 | 294 |
| Hb9 | AGCTGGGCGCCGGCACCTTCC | CCGCCGCCGCCCTTCTGTTTCTC | 65°C | 37 | 348 |
| HoxC9 | GGCAGCAAGCACAAAGAGGAG | GCAGGCTGGGTAGGGTTTAGG | 55°C | 40 | 301 |
| Irx3 | TGTCCAACGTGCTCTCGTC | TCCTCCTCGTCCTCCTCTTC | 62°C | 37 | 517 |
| Isl1 | AGGATGTGGAGGTAGTGAGA | GGAGATCTCAGTGGCTCTT | 54°C | 40 | 356 |
| Lim3 | TCAAGCGCTTCGGGACCAAG | TCCTGAACGCTGTCCTTGTCC | 60°C | 40 | 493 |
| Ngn2 | GAAGACCCGTAGACTGAA GGC | GAAAGGGAACCCACTAAGGC | 54°C | 40 | 537 |
| Nkx2.2 | TCTACGACAGCAGCGACAAC | GAACCAGATCTTGACCTGCG | 55°C | 40 | 335 |
| Olig2 | AAGCTAGGAGGCAGTGGCTTCAAGTC | CCGTCACCAGTCGCTTCATC | 60°C | 28 | 312 |
| Otx2 | ACGACGTTCACTCGGGCGCAG | ACTGCTGCTGGCAATGGTCGG | 58°C | 40 | 312 |
| Pax3 | CACTCGCCTTTCCGTTTCGCC | CGTTGTCACCTGCTTGGGCTTGC | 58°C | 40 | 393 |
| Pax6 | GGCAACCTACGCAAGATGGC | TGAGGGCTGTGTCTGTTCGG | 62°C | 35 | 459 |
| Pax7 | ACGGCTGCGTCTCCAAGATTC | GCTTCAGTGGGAGGTCAGGTTC | 58°C | 40 | 405, 399 |
| Phox2b | GTCCAAGTGGAAAGAGCCAAG | CCGTGGTCCGTGAAGAGTTTG | 61°C | 40 | 495 |
| Sox2 | TACCTCTTCCTCCCACTCCA | ACTCTCCTCTTTTGCACCCC | 55°C | 35 | 269 |