Table 2.
Antibodies used in immunostaining and immunoblotting.
| Name of antibody | Type | Vendor | Dilution |
|---|---|---|---|
| Immunostaining: | |||
| β3-tubulin | Mouse monoclonal | Covance | 1:5000 |
| BrdU | Mouse monoclonal | Sigma | 1:1000 |
| ChAT | Goat polyclonal | Chemicon | 1:50–100 |
| GABA | Rabbit polyclonal | Sigma | 1:1000 |
| GFAP | Rabbit polyclonal | Chemicon | 1:1000–2000 |
| Glutamate | Rabbit polyclonal | Sigma | 1:4000 |
| Hb9 | Mouse monoclonal | Developmental Studies Hybridoma Bank | 1:50–200 |
| MAP2 | Rabbit polyclonal | Chemicon | 1 :1000 |
| Olig2 | Goat polyclonal | R&D Systems | 10 ug/ml |
| O4 | Mouse monoclonal | R&D Systems | 1:500 |
| Immunoblotting: | |||
| β-actin | Mouse monoclonal | Sigma | 1:25000 |
| AKT | Rabbit polyclonal | Cell Signaling Technology | 1:500 |
| Phospho-AKT (Ser473) | Rabbit polyclonal | Cell Signaling Technology | 1:500 |
| p44/42 MAP Kinase | Rabbit polyclonal | Cell Signaling Technology | 1:500 |
| Phospho-p44/42 MAP Kinase (Thr202/Tyr204) | Rabbit polyclonal | Cell Signaling Technology | 1:500 |
| SAPK/JNK | Rabbit polyclonal | Cell Signaling Technology | 1:1000 |
| phospho-SAPK/JNK (Thr183–Tyr185) | Rabbit monoclonal | Cell Signaling Technology | 1:1000 |
BrdU, bromodeoxyuridine; ChAT, choline acetyltransferase; GABA, gamma-aminobutyric acid; GFAP, glial fibrillary acidic protein; MAP2, microtubule-associated protein 2.