. 2009 Jul 6;10(7):3012–3032. doi: 10.3390/ijms10073012

Table 1.

Identification of secreted proteins from 2-DE analysis of culture medium. Identified proteins together with the corresponding organism, accession number, peptides, theoretical isoelectric point (pI), molecular weight (Mw), and annotation of signal peptides are listed. Molecular weight and pI were not available for identified EST-clones. When, database searches lead to the identification of the same protein for several spots data were summarized. Individual databases are indicated as a) TREMBL, b) TIGR and c) Swissprot. Annotations for signal peptide were estimated from N-terminal sequences (70 aa) with SignalP 3.0 (http://www.cbs.dtu.dk/services/SignalP) and the following settings: organism was eukaryotes and method was hidden Markov model with a positive result at a probability higher than 0.55).

Spot	Description	Organism	Accession	Peptides	mW	pI	Signal
Hydrolyzing O-glycoside
1, 2, 3, and 4	Xylan 1,4 beta xylosidase ^a	P. tremula	Q2MCJ5	(K)AVSNNFATLMR(L)	81423	6,0	yes
	LEXYL2 protein Fragment ^a	S. lycopersicum	Q76MS4	(K)VTQQDLDDTFNPPFK(S)	68868	7,7	no
	LEXYL2 protein Fragment ^a	S. lycopersicum	Q76MS4	(K)VDMTNMNMR(A)
	homologue to LEXYL2 protein Fragment Q76MS4 ^b	S. tuberosum	TC145815	(T)AVSNNFATLMR(T)			no
				(T)VTQQDLDDTFNPPFK(C)
				(T)AVSNNFATLMR(T) Ox (M10)
	similar to Q76MS4 LEXYL2 protein Fragment partial 37 ^b	N. tabacum	TC13056	(A)LPMTWYPQSYADK(T)
				(A)LPMTWYPQSYADK(T) Ox (M3)
				(G)VDMTNMNMR(G)
				(A)GPTVFNFGDGLSYSNYK(C)
12, 14, 15, 22	Xylan 1,4 beta xylosidase ^a	P. tremula	Q2MCJ5	(K)AVSNNFATLMR(L)	81423	6,0	Yes
18, 20, 21,	Beta xylosidase like protein ^a	A. thaliana	Q9LXD6	(R)LGFFDGDPK(S)	83168	7,2	yes
22, and 23	LEXYL2 protein Fragment ^a	S. lycopersicum	Q76MS4	(K)VTQQDLDDTFNPPFK(S)	68868	7,7	no
	homologue to Q76MS4 LEXYL2 protein Fragment partial 88 ^b	S. tuberosum	TC145815	(C)AVSNNFATLMR(G)			no
	homologue to Q76MS4 LEXYL2 protein Fragment partial 88 ^b	S. tuberosum	TC145815	(T)VTQQDLDDTFNPPFK(C)
	similar to Q76MS4 LEXYL2 protein Fragment partial 37 ^b	N. tabacum	TC13056	(C)LPMTWYPQSYADK(T)
25	Alpha galactosidase Fragment ^a	Petunia hybrida	Q6UAY5	(K)TFASWGVDYLK(Y)	31305	4,7	no
27	LEXYL2 protein Fragment ^a	S. lycopersicum	Q76MS4	(K)VTQQDLDDTFNPPFK(S)	68868	7,7	no
	Xylan 1,4 beta xylosidase ^a	P. tremula	Q2MCJ5	(K)AVSNNFATLMR(L)	81423	6,0	yes
	homologue to Q76MS4 LEXYL2 protein Fragment partial 88 ^b	S. tuberosum	TC145815	(T)VTQQDLDDTFNPPFK(C)			no
	homologue to Q76MS4 LEXYL2 protein Fragment partial 88 ^b	S. tuberosum	TC145815	(T)AVSNNFATLMR(T)
34	Glucan endo 1,3 beta glucosidase acidic isoform PR Q precursor ^c	N. tabacum	P36401	(R)IYDPDQPTLEALR(G)	36971	5,0	yes
				(R)YIAVGNEVSPLNENSK(Y)
				(K)YVPVLLNAMR(N)
				(R)NIQTAISGAGLGNQIK(V)
				(K)VSTAIETGLTTDTSPPSNGR(F)
35	Alpha galactosidase ^a	C. canephora	Q5DUH7	(K)LGIYSDAGTQTCSK(T)	41203	5,5	no
37, 38	Xyloglucan endotransglucosylase hydrolase XTH3 ^a	S. lycopersicum	Q6RHY0	(R)IIFYVDGTPIR(E)	32134	5,1	yes
	homologue to Q6RHY0 Xyloglucan endotransglucosylase ^b hydrolase XTH3 partial	N. tabacum	TC5192	(A)IIFYVDGTPIR(T)			yes
		N. tabacum	TC5192	(C)APFSASYR(A)
	homologue to Q6RHY0 endotransglucosylase hydrolase XTH3 ^b partial	S. lycopersicum	TC186880	(G)NSESIGVSYPK(T)			yes
41	LEXYL2 protein Fragment ^a	S. lycopersicum	Q76MS4	(K)VTQQDLDDTFNPPFK(S)	68868	7,7	no
	homologue to Q76MS4 LEXYL2 protein Fragment partial 88 ^b	S. tuberosum	TC145815	(T)VTQQDLDDTFNPPFK(C)			no
	homologue to Q76MS4 LEXYL2 protein Fragment partial 88 ^b	S. tuberosum	TC145815	(T)AVSNNFATLMR(T)
52	LEXYL2 protein Fragment complete ^b	S. lycopersicum	TC170394	(T)ISMLVNTAGSVSR(T)			no
57	Glucan endo 1 3 beta glucosidase acidic isoform PR Q ^c	N. tabacum	P36401	(R)IYDPDQPTLEALR(G)	36971	5,0	yes
Protease acitivity
57	Subtilisin like protease ^a	N. tabacum	A9XG40	(R)TPSFLGLDR(S)	81136	6,6	yes
	Subtilisin like protease ^a	N. tabacum	A9XG40	(K)GYETTLGPVDVSK(E)
	Subtilisin like protease ^a	N. tabacum	A9XG41	(R)TPSFLGLDR(S)
	Subtilisin like protease ^a	N. tabacum	A9XG41	(K)GYETTLGPVDVSK(E)	81123	6,7	yes
6	Subtilisin like protease ^a	N. tabacum	A9XG40	(K)GYETTLGPVDVSK(E)	81136	6,6	yes
				(K)VSTVFSPSNSVK(V)
				(K)VSVEPETLVFTR(A)
	Subtilisin like protease ^a	N. tabacum	A9XG41	(K)GYETTLGPVDVSK(E)	81123	6,7	yes
				(K)VSTVFSSSNSVK(V)
				(K)VSVEPETLVFTR(V)
Chitinases activity
45, 46,	CHIP TOBAC Acidic endochitinase P precursor ^c	N. tabacum	P17513	(R)GPIQLTNQNNYEK(A)	27452	4,7	yes
45, 46,	CHIP TOBAC Acidic endochitinase P precursor ^c	N. tabacum	P17513	(R)QDLVNNPDLVATDATISFK(T)
47	Chitinase ^a	M. charantia	Q207U1	(R)SFPGFGTTGDDATR(K)	34034	8,1	yes
	CHIQ TOBAC Acidic endochitinase Q precursor ^c	N. tabacum	P17514	(R)WTPSAADQAANR(V)	27615	4,9	yes
	CHIP TOBAC Acidic endochitinase P precursor ^c	N. tabacum	P17513	(R)GPIQLTNQNNYEK(A)	27452	4,7	yes
Peroxidase activity
11	Peroxidase ^a	P. sativum	Q18PR1	(R)ISPLTGTNGEIR(K)	34257	8,2	yes
	Peoxidase like protein ^a	A. thaliana	Q0WLG9	(K)DAAPNANSAR(G)	38084	5,6	yes
	similar to Q9LEH3 Peroxidase precursor partial 91 ^b	N. tabacum	TC12826	(T)GFDVVDNIK(A)			yes
24	Lignin forming anionic peroxidase ^c	N. tabacum	P11965	(K)DAPANVGAGGFDIVDDIK(T)	34652	4,5	yes
				(K)GPSWQVLFGR(K)
				(K)GMDLTDLVALSGAHTFGR(A) Ox (M2)
				(K)LGNISPLTGTNGQIR(T)
Protein binding and Proteinase inhibitor activity
36	Biotic cell death associated protein ^a	N. glutinosa	Q850R9	(K)IEDGFVTTGGIK(G)	25141	8,6	yes
49	Biotic cell death associated protein ^a	N. glutinosa	Q850R9	(K)IEDGFVTTGGIK(G)	25141	8,6	yes
Pathogenesis related protein family 1 – unknown function
55, 58	Pathogenesis related protein 1B ^c	N. tabacum	P07053	(K)AVEMWVDEK(Q)	18487	5,2	yes
	Pathogenesis related protein 1B ^c	N. tabacum	P07053	(K)AVEMWVDEK(Q) Ox (M4)
	Pathogenesis related protein 1A ^c	N. tabacum	P08299	(K)AVEMWVDEK(Q)	18561	4,6	yes
	Pathogenesis related protein 1A ^c	N. tabacum	P08299	(K)AVEMWVDEK(Q) Ox (M4)
	Pathogenesis related protein 1C ^c	N. tabacum	P09042	(K)AVEMWVNEK(Q)	18570	5,2	yes