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. Author manuscript; available in PMC: 2010 Feb 15.

Published in final edited form as: Transplantation. 2009 Feb 15;87(3):360–369. doi: 10.1097/TP.0b013e31819574e9

CXCR3 antagonism does not inhibit infiltration of primed effector CD8 T cells into cardiac allografts. A. B6.Thy1.1 mice received A/J heart grafts and primed CD8 effectors were purified from the spleens on day 7 post-transplant. 5×10⁶ of these Thy1.1⁺ effectors were transferred to B6.Thy1.2 recipients of A/J heart grafts on day +2. B6.Thy1.2 recipients were treated with vehicle or AMG1237845, grafts were harvested on day +4, and infiltrating cells were subjected to flow cytometry analysis. B. Surface marker expression analysis by flow cytometry of naïve and primed Thy1.1⁺ cells. C. CXCR3 expression on primed effector (CD44^hi) and naïve (CD44^lo) CD8⁺ T cells. D. Quantification of infiltrating Thy1.1⁺CD8⁺ T cells in vehicle- and AMG1237845-treated B6 recipients of A/J grafts, B6 recipients of DBA grafts, and naïve Thy1.1 infiltration in B6 recipients of A/J grafts (n = 3-4/group). E. Surface expression of CXCR3 on transferred effectors in recipient spleen and in the grafts at the time of harvest (representative of 4/group; Isotype, CXCR3).

Inline graphic — CXCR3 antagonism does not inhibit infiltration of primed effector CD8 T cells into cardiac allografts. A. B6.Thy1.1 mice received A/J heart grafts and primed CD8 effectors were purified from the spleens on day 7 post-transplant. 5×10⁶ of these Thy1.1⁺ effectors were transferred to B6.Thy1.2 recipients of A/J heart grafts on day +2. B6.Thy1.2 recipients were treated with vehicle or AMG1237845, grafts were harvested on day +4, and infiltrating cells were subjected to flow cytometry analysis. B. Surface marker expression analysis by flow cytometry of naïve and primed Thy1.1⁺ cells. C. CXCR3 expression on primed effector (CD44^hi) and naïve (CD44^lo) CD8⁺ T cells. D. Quantification of infiltrating Thy1.1⁺CD8⁺ T cells in vehicle- and AMG1237845-treated B6 recipients of A/J grafts, B6 recipients of DBA grafts, and naïve Thy1.1 infiltration in B6 recipients of A/J grafts (n = 3-4/group). E. Surface expression of CXCR3 on transferred effectors in recipient spleen and in the grafts at the time of harvest (representative of 4/group; Isotype, CXCR3).