CXCR3 blockade decreases the number of donor-reactive, IFN-γ-producing CD8+ T cells in the recipient spleen but does not alter IFN-γ expression in the graft. A. CD8+ T cells from naïve B6 mice, vehicle-, and AMG1237845-treated allograft recipient mice were purified on day 7 post-transplant (n = 4/group) and were subjected to IFN-γ ELISPOT. Representative data from one of three repeated experiments is shown. B. mRNA was purified from isografts, vehicle-, and AMG1237845-treated allografts on day 7 post-transplant. Quantitative RT-PCR results for IFN-γ and the IFN-γ-inducible chemokine CXCL9 were normalized to isograft expression levels. There was no significant difference in expression of either gene between vehicle-treated and AMG1237845-treated allograft groups at the 95% confidence interval.