Figure 8. Overexpression of m-aconitase does not lead to cell death of astrocytes.

Primary midbrain cultures were transduced with AdAcon for 24–48 hrs and GFP fluorescence (green) was observed. Representative images of (A) neurons detected by MAP2 staining (red) and (B) astrocytes detected by GFAP staining (red). (A,B) Arrows point to neurons and astrocyte, respectively. (C) AdGFP and AdAcon transduced astrocytes were labeled with GFAP (red) after 18 hrs of 1000 µM PQ²⁺ incubation. Nuclei (blue) were counterstained with DAPI, representative images, 40× magnification.