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. Author manuscript; available in PMC: 2010 Sep 15.
Published in final edited form as: J Neurosci Methods. 2009 Jun 21;182(2):236–243. doi: 10.1016/j.jneumeth.2009.06.015

Fig. 4.

Fig. 4

Time course of 13C-labeled glutamate C5, glutamine C5, and GABA C1 concentrations (μmol/g) due to acute gabaculine administration and isotope chase by infusing unlabeled glucose. The inset depicts the treatment protocol starting with the initial 2.5 hr infusion of [2,5-13C2]glucose, followed by gabaculine administration and isotope chase 2 hr after gabaculine administration using natural abundance glucose. Quantification was based on high-resolution in vitro NMR analysis of brain perchloric acid extracts at the end point. The end point GABA C1 concentration was 4.5 ± 0.3 μmol/g (n = 5, mean ± SD), corresponding to an isotopic fractional enrichment of 49.7 ± 2.6% (n = 5, mean ± SD).