Table 2.
Primer sequence for RT-PCR
| Gene name | Primer sequence | Seqence ID | Cycle # | Productsize (bp) |
|---|---|---|---|---|
| Cre | F: GCATTACCGGTCGATGCAACGAGTG | AF234173.1 | 30 | 374 |
| B: GAACGCTAGAGCCTGTTTTGCACGTTC | ||||
| PPARγ | F: TTCTCAGACTCGAAGCAGCA | NM_013672 | 35 | 346 |
| B: CACAACATACTGCCCACCAG | ||||
| SOD1 | F: AGGCTGTACCAGTGCAGGAC | X 06683 | 28 | 300 |
| B: GTTTACTGCGCAATCCCAAT | ||||
| CAT | F: CCTCGTTCAGGATGTGGTTT | X 52108 | 32 | 325 |
| B: GGCATCCCTGATGAAGAAAA | ||||
| GST | F: AAGACTGCCTTGGCAAAAGA | NM_029555 | 26 | 345 |
| B: GCCAGTATCTGTGGCTCCAT | ||||
| UCP-1 | F: TCTCAGCCGGCTTAATGACT | NM_009463 | 32 | 445 |
| B: GTCGGTCCTTCCTTGGTGTA | ||||
| LXRα | F: GGATAGGGTTGGAGTCAGCA | NM_013839 | 26 | 347 |
| B: GCAGGACTTGAGGAGGTGAG | ||||
| LPL | F: GCTGGCCTTGAACTCTCAAC | NM_008509 | 28 | 379 |
| B: CGGCAACAATCCTGGTACTT | ||||
| GAPDH | F: TGTTCCTACCCCCAATGTGT | NM_001001303 | 22 | 396 |
| B: TGTGAGGGAGATGCTCAGTG | ||||
| SYNP | F: AGACAGGCAGGTGAAGAGGA | NM_009305 | 20 | 483 |
| B: GGGCCACAGTCAGACAAAAT | ||||
| EAAT2 | F: TCTGAGGAGGCCAATACCAC | NMMU11763 | 30 | 312 |
| B: GTCCTTGATGGCGATGATCT |
F, Forward; B, backward.