Abstract
The hemagglutination inhibition test (HAI) and the enzyme-linked immunosorbent assay (ELISA) for detecting antibody to rubella virus were compared by testing 25 sets of paired sera taken before and after infection and 10 sets of sera taken during acute and convalescent stages of the disease and by screening 700 serum samples from the Collaborative Perinatal Project, NIH/NINCDS. The tests were found to be comparable in their ability to detect positive and negative sera, rises in titers, and seroconversions. When a purified antigen and carefully prepared reagents were used, ELISA was found to be as accurate and reliable as HAI. ELISA required no pretreatment of serum, could easily be automated, and was less time-consuming than HAI.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Buimovici-Klein E., O'Beirne A. J., Millian S. J., Cooper L. Z. Low level rubella immunity detected by ELISA and specific lymphocyte transformation. Arch Virol. 1980;66(4):321–327. doi: 10.1007/BF01320628. [DOI] [PubMed] [Google Scholar]
- Forghani B., Schmidt N. J. Antigen requirements, sensitivity, and specificity of enzyme immunoassays for measles and rubella viral antibodies. J Clin Microbiol. 1979 Jun;9(6):657–664. doi: 10.1128/jcm.9.6.657-664.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Garland S. M., Locarnini S. A., Gust I. D. A solid-phase enzyme-linked immunosorbent assay (ELISA) for detection of antibody to rubella virus. Pathology. 1979 Jul;11(3):393–399. doi: 10.3109/00313027909059017. [DOI] [PubMed] [Google Scholar]
- Kalimo K. O., Meurman O. H., Halonen P. E., Ziola B. R., Viljanen M. K., Granfors K., Toivanen P. Solid-phase radioimmunoassay of rubella virus immunoglobulin G and immunoglobulin M antibodies. J Clin Microbiol. 1976 Aug;4(2):117–123. doi: 10.1128/jcm.4.2.117-123.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leinikki P. O., Shekarchi I., Dorsett P., Sever J. L. Enzyme-linked immunosorbent assay determination of specific rubella antibody levels in micrograms of immunoglobulin G per milliliter of serum in clinical samples. J Clin Microbiol. 1978 Oct;8(4):419–423. doi: 10.1128/jcm.8.4.419-423.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leinikki P., Pässilä S. Solid phase antibody assay by means of enzyme conjugated to anti-immunoglobulin. J Clin Pathol. 1976 Dec;29(12):1116–1120. doi: 10.1136/jcp.29.12.1116. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
- Sever J. L., Hardy J. B., Nelson K. B., Gilkeson M. R. Rubella in the collaborative perinatal research study. II. Clinical and laboratory findings in children through 3 years of age. Am J Dis Child. 1969 Jul;118(1):123–132. doi: 10.1001/archpedi.1969.02100040125020. [DOI] [PubMed] [Google Scholar]
- Sever J. L. Rubella serology: a need for improvement. Obstet Gynecol. 1980 Jul;56(1):127–128. [PubMed] [Google Scholar]
- Taylor R. N., Fulford K. M., Przybyszewski A., Pope V. Center for disease control diagnostic immunology proficiency testing program results for 1976. J Clin Microbiol. 1977 Sep;6(3):224–232. doi: 10.1128/jcm.6.3.224-232.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor R. N., Fulford K. M., Przybyszewski V. A., Pope V. Center for Disease Control Diagnostic Immunology Proficiency Testing Program results for 1977. J Clin Microbiol. 1978 Oct;8(4):388–395. doi: 10.1128/jcm.8.4.388-395.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor R. N., Fulford K. M., Przybyszewski V. A., Pope V. Center for Disease Control Diagnostic Immunology Proficiency Testing Program results for 1978. J Clin Microbiol. 1979 Dec;10(6):805–814. doi: 10.1128/jcm.10.6.805-814.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]