Fig. 8.

On-chip fluorescent detection systems for μFFE. (a) A laser line is projected across the width of the separation channel for excitation. Fluorescent signal is imaged by microscope and recorded by a CCD camera. (b) Similar setup to (a), but excitation is done by light projected though the microscope optics. (c) A laser dot on the chip excites a small portion of the separation channel. Fluorescent signal is recorded using a photomultiplier tube. A translating stage scans the chip through the detection window to acquire data across the width of the separation channel