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. Author manuscript; available in PMC: 2009 Sep 7.
Published in final edited form as: DNA Repair (Amst). 2007 Jun 7;6(10):1413–1424. doi: 10.1016/j.dnarep.2007.04.004

Figure 4. E. coli show enhanced RecA and RecB-independent inaccurate end-joining of linear DNA when both Mt-Ku and Mt-LigD are expressed.

Figure 4

Wild-type (BW35) bacteria and strains capable of expressing Mt-Ku (BWKu), Mt-LigD (BWLig) or Mt-Ku and Mt-LigD (BWKuLig#1 and #2, A−KuLig and B−KuLig) were grown in LB or LB supplemented with 0.2% L-arabinose prior to the preparation of electrocompetent bacteria. Bacteria were then co-transformed with 100 ng PacI-linearized pBestluc and 0.1 ng pACYC184. A ratio of CarbR to CmR colonies was calculated for each transformation for the total number of CarbR colonies (A – Total Repair), as well as the CarbR colonies expressing active luciferase (B – Accurate Repair) or inactive luciferase (C – Inaccurate Repair). At least seven transformations were performed and the average and standard error are shown.