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. Author manuscript; available in PMC: 2009 Sep 7.
Published in final edited form as: DNA Repair (Amst). 2007 Jun 7;6(10):1413–1424. doi: 10.1016/j.dnarep.2007.04.004

Figure 5. DNA end-joining of ClaI-digested DNA by Mt-Ku and Mt-LigD in wild-type E. coli.

Figure 5

BWKuLig#1 was grown in LB or LB supplemented with 0.2% L-arabinose prior to the preparation of electrocompetent bacteria. Bacteria were co-transformed with 100 ng ClaI-linearized pBestluc and 0.1 ng pACYC184. A ratio of CarbR to CmR colonies was calculated for each transformation for the total number of CarbR colonies (Total Repair), as well as the CarbR colonies expressing active luciferase (Accurate Repair) or inactive luciferase (Inaccurate Repair). Nine transformations were performed and the average and standard error are shown.