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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Nat Neurosci. 2009 Jun 14;12(7):864–871. doi: 10.1038/nn.2346

Figure 5. Active JNK3 mimics the effect of polyQ-Htt on FAT.

Figure 5

Effects of active, recombinant JNK1, JNK2 and JNK3 were evaluated by vesicle motility assays in isolated axoplasm. A) Perfusion of active JNK1 kinase (200nM) in axoplasm had no effect on FAT. B) Perfusion of active JNK2 (100nM) slightly inhibited anterograde FAT, but had no significant effect on retrograde FAT. C) Perfusion of JNK3 (100nM) significantly inhibited FAT in both directions, as observed with polyQ-Htt (compare to Fig. 2B) D) Bar graphs showing mean FAT rates in axoplasm perfused with active JNKs and polyQ-Htt. Error bars show SEM. Data represent pooled FAT rate measurements between 30-50 min of observation. Anterograde FAT rates after polyQ-Htt perfusion were comparable to those elicited by perfusion of active JNK3 and both JNK3 and polyQ-Htt significantly inhibit retrograde FAT.