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. Author manuscript; available in PMC: 2010 Jun 20.
Published in final edited form as: J Comp Neurol. 2009 Jun 20;514(6):567–582. doi: 10.1002/cne.22052

Figure 1.

Figure 1

GCV administration suppresses formation of neurospheres (NS) derived from P40 nestin-tk mouse SVZ. A: This schematic of the nestin-tk construct shows that expression of the HSV-tk gene is under control of a nestin 2nd intronic enhancer element and minimal tk promoter. Administration of GCV to mice carrying this transgene results in death of dividing cells expressing HSV-tk. B: Culture of NS in GCV-containing media for 24 hours or 5 days results in decreased NS formation from nestin-tk+ (tk+) mice relative to GCV-treated wild-type controls (wt; p < 0.001) or tk+ NS cultures in basal media (p < 0.001) after 5 days in vitro. Black bars indicate number of large (L), medium-sized (M) and small (S) NS derived from wt mice, while gray bars denote those derived from tk+ mice. C–H: Representative phase contrast images of cultured NS after 5 day in vitro. NS from tk+ mice cultured in GCV-containing media are markedly reduced (G, H) relative to those cultured in control media (F) or to cultures from wt controls grown in the presence or absence of GCV (C–E). Scale bar = 100 μm.