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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Arch Biochem Biophys. 2009 May 22;487(1):10–18. doi: 10.1016/j.abb.2009.05.008

Figure 6.

Figure 6

Reaction of oxidized PDO (15 μM) with a stoichiometric amount of reduced PDR ([PDO Rieske]:[PDR] = 1:1) in 0.1M HEPES pH 7.8, in the presence of 3 mM phthalate at 22 °C. Experimental traces (solid line) and fits obtained with the parallel reaction model (dotted lines) for K117A (1), WT (2) and W94F variant (3). Traces are offset from each other by 0.01 absorbance units for clarity. All concentrations are those before mixing in the stopped-flow spectrophotometer. Presented traced were recorded at 640 nm.