. Author manuscript; available in PMC: 2010 Jul 1.

Published in final edited form as: Arch Biochem Biophys. 2009 May 22;487(1):10–18. doi: 10.1016/j.abb.2009.05.008

Table 1.

Primers used for the construction of PDO mutants.

Mutation	Primers
PDO: Lys117Ala	GTAGCATGACCGACGCGGTCAAGCACAAGGCC
PDO: Lys117Ala	GGCCTTGTGCTTGACCGCGTCGGTCATGCTAC
PDO: Lys119Ala	GACCGACAAGGTCGCGCACAAGGCCTATCCC
PDO: Lys119Ala	GGGATAGGCCTTGTGCGCGACCTTGTCGGTC
PDO: His120Ala	CGACAAGGTCAAGGCCAAGGCCTATCCCGTGC
PDO: His120Ala	GCACGGGATAGGCCTTGGCCTTGACCTTGTCG
PDO: Lys121Ala	ACCGACAAGGTCAAGCACGCGGCCTATCCCG
PDO: Lys121Ala	CGGGATAGGCCGCGTGCTTGACCTTGTCGGT
PDO: Tyr123Ala	TCAAGCACAAGGCCGCTCCCGTGCAGGAATGG
PDO: Tyr123Ala	CCATTCCTGCACGGGAGCGGCCTTGTGCTTGA
PDO: 5 Point Mutation	GCATGACCGACGCGGTCGCGGCCGCGGCCGCTCCCGTGCAGGAA
PDO: 5 Point Mutation	TTCCTGCACGGGAGCGGCCGCGGCCGCGACCGCGTCGGTCATGC
PDO: Trp94Tyr	CTGCGCTGCCTGTATCACGGCTATAAGTTTGACGTCG
PDO: Trp94Tyr	CGACGTCAAACTTATAGCCGTGATACAGGCAGCGCAG
PDO: Trp94Phe	GCGCTGCCTGTATCACGGCTTCAAGTTTGACGTC
PDO: Trp94Phe	GACGTCAAACTTGAAGCCGTGATACAGGCAGCGC
PDO: Trp94Ala	GCTGCCTGTATCACGGCGCGAAGTTTGACGTC
PDO: Trp94Ala	GACGTCAAACTTCGCGCCGTGATACAGGCAGC
PDR: 6 HIS insertion	5′-CCGCAGAGCTGGTTCTCGACCTTCACCACCACCACCACCACTAAGTATCGCGGAGCAAAC-3′
PDR: 6 HIS insertion	5′-GTTTGCTCCGCGATACTTAGTGGTGGTGGTGGTGGTGAAGGTCGAGAACCAGCTCTGCGC-3′