Table 5.

Rates of Rieske center reduction by sodium dithionite in PDO WT and the mutants.

	Rieske center reduction*
	k1, s−1	A1 (%)	k2, s−1	A2 (%)
WT	0.12	10	1.0 10−3	90
K117A	0.24	2	0.8 10−3	98
K119A	0.31	13	4.2 10−3	87
H120A	0.27	8	1.6 10−3	92
K121A	n.d. **	n.d.	3.6 10−3	100
Y123A	0.48	9	2.2 10−3	91
5 point mutation	0.32	6	1.6 10−3	94
W94Y	3.1	2	0.012	98
W94F	n.d.	n.d.	0.040	100
W94A	5.9	100	n.d.	n.d.

^*An anaerobic solution of PDO (30–40 μM) in 0.1M HEPES, pH 7.8 in the presence of 3 mM phthalate was mixed with anaerobically prepared 2 mM sodium dithionite in the same buffer. Concentrations are presented before mixing.

^**n.d. – phase not detected