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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Int J Oncol. 2009 Oct;35(4):851–859. doi: 10.3892/ijo_00000399

Figure 1. RNAi-mediated downregulation of MMP-2 causes reduction of MMP-2 mRNA expression and protein activity, activation of caspase-8, and overexpression of NIK.

Figure 1

Human glioma xenograft cells 4910 were infected with Ad-SV (10 MOI) or Ad-MMP-2 (2.5, 5 or 10 MOI) for 36 hrs. MMP-2 mRNA expression was determined by RT-PCR analysis after 48 hrs of adenovirus infection; the experiment was performed in triplicate (A). For the control, we used uninfected cells or cells infected with 10 MOI of Ad-SV. The expression levels were determined by image densitometry (B). Human glioma xenograft cells 4910 were infected with Ad-SV (10 MOI) or Ad-MMP-2 (2.5, 5 or 10 MOI) for 48 hrs. Expression levels of cleaved caspase-8, TRADD, TRAF-2 and NIK were determined by western blot analysis (A). Expression levels of GAPDH were also determined and served as loading controls. Quantitative analysis of the expression levels of cleaved caspase-8, TRADD, TRAF-2 and NIK were determined by densitometry. All experiments were done in triplicate (B).