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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Int J Oncol. 2009 Oct;35(4):851–859. doi: 10.3892/ijo_00000399

Figure 2. MMP-2 downregulation causes the nuclear translocation of AIF.

Figure 2

4910 glioma xenograft cells (1×103) were seeded in 8-well chamber slides, incubated for 24 hrs, and then incubated with Ad-SV (10 MOI) or Ad-MMP-2 (2.5, 5 or 10 MOI) for 36 hrs. Next, cells were fixed and immunoprobed for AIF using a specific monoclonal antibody as per standard protocols. HRP-conjugated secondary antibody was used to visualize AIF in 4910 cells in conjunction with a DAB reagent (A). Nuclear localization of AIF was also determined over time at 6, 12, 24, 48 and 72 hrs post-infection in triplicate (B).