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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Int J Oncol. 2009 Oct;35(4):851–859. doi: 10.3892/ijo_00000399

Figure 3. MMP-2 downregulation causes the nuclear translocation of AIF and the dephosphorylation of pIκbα.

Figure 3

Human glioma xenograft cells 4910 were infected with Ad-SV (10 MOI) or Ad-MMP-2 (2.5, 5 or 10 MOI) for 36 hrs. After infection, cells were collected and separated into nuclear and cytoplasmic fractions and proteins extracted as per standard protocols. Expression levels of AIF and pIκbα were determined by western blot analysis (A). Levels of nuclear localization were quantified in triplicate by densitometry (B).