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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Int J Oncol. 2009 Oct;35(4):851–859. doi: 10.3892/ijo_00000399

Figure 6. MMP-2 downregulation induces the overexpression of caspase-8 mRNA and the downregulation of TP73 mRNA.

Figure 6

We used the RT2² Profiler™ PCR Array to profile the expression of genes related to the apoptotic signaling pathway. Human glioma xenograft cells 4910 were infected with Ad-SV (10 MOI) or Ad-MMP-2 (2.5, 5 or 10 MOI) for 36 hrs. Total RNA was isolated from treated and control cells, and mRNA were further isolated using oligo dT conjugated paramagnetic particles. cDNA was synthesized from the purified mRNA as per standard protocol. Real time PCR was performed and Ct values were plotted and fold change determined by 2-ΔCt (experiment-control) (A). Scatter plot analysis was carried out to validate experimental results (B).