Table 3.
Strains and plasmids.
| Strains | Relevant features | References |
|---|---|---|
| DH5∀ | E. coli, ∀-complementation | Stratagene |
| NTL4 | A. tumefaciens C58, pTi less | (Luo et al., 2001) |
| BL21/DE3 | E. coli B Plac-gene 1 of bacteriophage T7 | (Studier et al., 1990) |
| KY2347 | E. coli MG1655, (clpPX-lon) 1196::cat | (Herman et al., 1998) |
| Plasmids | ||
| pPZP201 | Broad-host-range cloning vector, SpcR | (Hajdukiewicz et al., 1994) |
| pMCSG9 | PT7-his6-MBP-TEV, AmpR | (Donnelly et al., 2006) |
| pJZ358 | PT7-traR, AmpR | (Zhu and Winans, 1999) |
| pYC335 | Plac-traR in pPZP201 | (Chai and Winans, 2004) |
| pCEW250 | pBluescriptSK+ with consensus tra box at PtraI, KmR | (White and Winans, 2007) |
| pCEW260 | Consensus PtraI lacZ fusion, KmR | (White and Winans, 2007) |
| pAFM02 | traR cloned into pMCSG9 | This study |
| pUP-5M to 229Y | traR point mutants cloned into pYC335 | This study |
| pUP2-5M to 229Y | traR point mutants cloned into pAFM02 | This study |
| pUP3-5M to 229Y | traR point mutants cloned into pJZ358 | This study |