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. Author manuscript; available in PMC: 2009 Nov 28.
Published in final edited form as: Cell. 2008 Nov 20;135(5):933–947. doi: 10.1016/j.cell.2008.10.011

Figure 4. Dynamic monitoring of ER redox status and UPR activity during inositol starvation.

Figure 4

(A–F) Time courses of eroGFP ratio or UPR-RFP metric histograms in wild-type, ire1Δ, or hac1Δ cells starved for inositol at time, t=0. (G) Growth curves for the time courses of (A–F). (H–J) Snapshots of hac1Δ cell populations for eroGFP ratio vs. Cy5 signal at three time points. Mothers (m) have a high Cy5 signal (log2 Cy5 > 10.5) while daughters (d) have a low Cy5 signal (log2 Cy5 < 10.5). Time courses of eroGFP histograms for hac1Δ mothers (K) and daughters (L). The three time points of (H–J) are indicated in (L). Color represents percentage of cells at a given metric value and time point.