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. Author manuscript; available in PMC: 2009 Nov 28.
Published in final edited form as: Cell. 2008 Nov 20;135(5):933–947. doi: 10.1016/j.cell.2008.10.011

Figure 6. Analysis of ER oxidative folding, quality control, and UPR signaling mutants under stress.

Figure 6

(A, B) Median trajectories of wild-type or mutant cells (treated with DTT or Tm) through UPR-RFP metric and eroGFP ratio two-dimensional space. The trajectories begin at the last data point before stress addition, and conclude when the time course ends (signified by the arrow). Consecutive white circles within each trajectory line are separated by 30 minutes. Color denotes each mutant. (C, D) Growth curves for wild-type cells treated with DTT (2mM) or Tm (1µg/ml). (E, F) The first derivative of median UPR-RFP with respect to time, d(UPR-RFP)/dt, vs. time for wild-type and mutants treated with DTT or Tm. (G) Integrated eroGFP ratios over the time courses for each mutant normalized to wild-type and represented as heat maps ranging from blue (negative values) to yellow (positive values). Data for (A–G) are from Figure 3. (H) Histogram of ire1Δ cells expressing eroGFP, reconstituted with a 1NM-PP1-sensitized IRE1 allele, and treated with DMSO (black line), 30µM 1NM-PP1 (green line), DMSO and 1µg/ml Tm (red line), and 30µM 1NM-PP1 and 1µg/ml Tm (yellow line). Inset shows median eroGFP ratio change for each treatment. Error bars are SD of three independent experiments.