Fig. 5.

β1^-/- CD cells are unable to spread or signal in response to growth factors. (A) β1^flox/flox and β1^-/- CD cells were allowed to adhere to collagen I or vitronectin (both at 10 μg/ml) for 45 minutes. Cells were then incubated with or without FGF2, FGF10 or GDNF (all at 10 ng/ml) and after 1 hour they were stained with Rhodamine Phalloidin. (B-E) β1^flox/flox and β1^-/- CD cells were allowed to adhere to collagen I (B-D) or vitronectin (E) for 2 hours, after which they were treated with the growth factor designated for various times. The cells were then lysed and 20 μg of total cell lysates were analyzed by western blot for levels of activated and total FAK, ERK, p38 MAPK and Akt. A representative experiment is shown.