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. Author manuscript; available in PMC: 2009 Sep 7.
Published in final edited form as: Biosci Rep. 2009 Feb;29(1):57–70. doi: 10.1042/BSR20080094

Fig. 3. Effect of overexpression of Ets-1 on Npr1 promoter activity.

Fig. 3

Luciferase activity of Npr1 promoter constructs having wild-type (WT) and mutant (mut) Ets-1A and Ets-1B binding sites when transfected in (A) MMCs and (B) MA-10 cells. Ets-1 expression plasmid (250 ng) or empty plasmid pEVRF0 (-Ets-1) was cotransfected along with Npr1 promoter construct. The results were normalized for the transfection efficiency as relative to light units per Renilla luciferase activity. Values represent fold induction as compared with empty vector (−Ets-1). Western blot (WB) analysis of Ets-1 in transfected (C) MMCs and (D) MA-10 cells along with β-actin as a control. Bars in A and B represent the mean ± SE of three to four independent experiments in triplicates. **, p< 0.01; ***, p < 0.001 vs Ets-1-transfected wild type construct.