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. Author manuscript; available in PMC: 2009 Sep 7.
Published in final edited form as: Biosci Rep. 2009 Feb;29(1):57–70. doi: 10.1042/BSR20080094

Fig. 5. Ets-1-mediated regulation of NPRA expression and GC activity in MMCs and MA-10 cells.

Fig. 5

A) MMCs and B) MA-10 cells were transfected with and without Ets-1 expression plasmids. Forty-eight hours after transfection, cells were lysed and total protein was isolated and subjected to Western blot using antibodies directed against NPRA. β-actin was used as loading control. (C) and (D) shows the densitometry quantitation of NPRA mRNA levels in MMCs and MA-10 cells, respectively. Values represent the fold induction relative to empty vector-transfected controls. GC activity in the plasma membrane preparations of Ets-1 transfected (E) MMCs and (F) MA-10 cells was measured as described in the ‘Materials and Methods’ section. WB indicates Western blot, -Ets indicates transfection with empty vector (pEVRF0), and +Ets indicates transfection with Ets-1 expression plasmid. Representative results of Western blots from 3 experiments are shown. Bars in C, D, E, and F represent mean ± S.E. of three separate determinants in triplicates. **, p < 0.01; ***, p<0.001.