Skip to main content
. Author manuscript; available in PMC: 2009 Sep 8.
Published in final edited form as: J Biol Chem. 2005 May 31;280(31):28601–28609. doi: 10.1074/jbc.M501484200

Figure 9. Trisk 49 and Trisk 32 expression during differentiation of myoblasts.

Figure 9

Primary cultures of satellite cells were induced in differentiation. When myotubes formed, the cells were fixed and labeled with antibodies against titin and Trisk 49 (panels a, b and c), or IP3R and Trisk 32 (panels d, e and f). The nuclei were stained with TO-PRO 3 (blue labeling in panels c and f). Titin and Trisk 49 antibodies labeled only myotubes (aligned nuclei) and not myoblasts (isolated nuclei), and both presented a striated pattern. IP3R and Trisk 32 antibodies label the myotubes (multinucleated cells) and the myoblasts (isolated nuclei), with a diffuse punctuated pattern.