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. Author manuscript; available in PMC: 2010 Nov 13.
Published in final edited form as: Mol Cell Endocrinol. 2009 Jul 24;311(1-2):47–54. doi: 10.1016/j.mce.2009.07.014

Figure 5. Blockade of ERK attenuates the additive insulin effect on GnRH induced translation.

Figure 5

LβT2 cells were transfected with a bicistronic reporter gene encoding firefly luciferase and β-galactosidase translated by cap-dependent and -independent mechanisms, respectively for 24 h. Cells were then serum starved for 4 h and pretreated with either vehicle or 50 µM PD098059 (PD) to block MAPK activation for 30 min. Cells were further treated for 6 h with vehicle, 10 nM GnRH (GnRH), 10 nM insulin (Ins), GnRH + Insulin, 1n M PMA, or 1nM PMA + insulin. Cells were then harvested and assayed for reporter gene activity. The histogram shows the ratio of luciferase to β-galactosidase activity. (#) indicates significant differences (p<0.05) between pairs connected by solid lines. (*) indicates significant increase above the control vehicle treated group as determined by ANOVA and post-hoc evaluation using either Students T test or Dunnett’s comparison to control test as appropriate.