Figure 1. Cyanovirin-N (CV-N) blocks herpes simplex virus type-1 (HSV-1) entry into CHO-K1 cells expressing gD receptors.
In this experiment, β-galactosidase-expressing recombinant virus HSV-1 (KOS) HSV-1 gL86 (25 pfu/cell) was pre-incubated with CV-N at indicated concentration or mock treated with 1 × phosphate saline buffer (PBS) for 90 min at room temperature. After 90 min the virus was incubated with CHO-K1 cells expressing gD receptors: nectin-1 (A), HVEM (B) and 3-OST-3 expressing cells (C). After 6 hr, the cells were washed, permeabilized and incubated with ONPG substrate (3.0 mg/ml) for quantitation of β -galactosidase activity expressed from the encoded viral genome. The enzymatic activity was measured at an optical density of 410 nm (OD 410). In this and other figures each value shown is the mean of three or more determinations (± SD; standard deviation) from four independent experiments. Mock treated HSV-1 with PBS was used as a control.