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. 2009 Sep 18;4(9):e7090. doi: 10.1371/journal.pone.0007090

Figure 3. Association with PA enhances the direct interaction between PLD1 and μ2.

Figure 3

(A) Purified PLD1 immobilized with anti-PLD antibody (PLD) or with pre-immune serum (CTL) was incubated with purified GST alone or GST-μ2. (B) HeLa cells expressing vector, wild type, R304A, or R304K PLD1 were grown in DMEM containing 10% fetal bovine serum. Cells were lysed and immunoprecipitated with anti-PLD antibody. (C) Purified PLD1 immobilized with anti-PLD antibody was incubated with purified GST alone or GST-μ2 in the absence or presence of C6-PA at the indicated concentrations. (D) Vector, wild type, K898R, or R158Q PLD1 transiently expressed in HeLa cells was immunoprecipitated and incubated with purified GST-μ2 in the absence or presence of 20 nM of C6-PA.