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. 2009 Sep 18;4(9):e7090. doi: 10.1371/journal.pone.0007090

Figure 4. The PLD1-μ2 interaction is important for adaptor recruitment onto membranes.

Figure 4

(A) Purified GST-fusion PLD1 296–312 or PLD1 296–312 (R304A) was added to recruitment assay mixture containing membrane preparation from wild type PLD1-expressing HeLa cells and rat brain cytosol (10 mg/ml). Membrane-recruited μ2 was detected by western blotting. Data are representative of two independent experiments. (B) Translocation of endocytic proteins onto plasma membrane was analyzed. HeLa cells transfected with vector, wild type, K898R, R158Q, or R304A PLD1 were treated with EGF (20 nM) during 2 min and the membrane preparations were subjected to SDS-PAGE and immunoblotted using the indicated antibodies.