Table 1.
Plasmids and oligonucleotides used.
| Plasmids or oligonucleotides | Description | Source/reference |
|---|---|---|
| Plasmida | ||
| (1) pBluescript KS II (+) | pKS cloning vector | Stratagene |
| pKS:F09E10.31 | F09E10.3 in pBluescript | This study |
| pKS:D1054.81 | D1054.8 in pBluescript | This study |
| (2) YEp352 | URA3-marked multicopy plasmid | [3] |
| (3) YEp:CTA12 | CTA1 behind its own promoter | [4] |
| YEp:F09E10.33 | F09E10.3 behind the CTA1 promoter | This study |
| YEp:D1054.83 | D1054.8 behind the CTA1 promoter | This study |
| YEp:OAR13 | OAR1 behind the CTA1 promoter | Z.-J. Chen |
| oligonucleotideb | ||
| Ce OAR1-1 5′ XbaI | TTATTCTAGATGAGTGCTTTGCTGGGAGG | This study |
| Ce OAR1-1 3′ XhoI | TATTCTCGAGCTACATACTAAATCCACCGG | This study |
| Ce OAR1-2 5′ XbaI | TTATTCTAGATGACTCGTTTTGCTGAAAAAGTC | This study |
| Ce OAR1-2 3′ XhoI | TATTCTCGAGTTAATGTAAAAGTTTTGCAAAG | This study |
aThe superscripts following the plasmid designations refer to their parental vectors, for example, pKS:F09E10.31 was derived from (1) pBluescript KS II (+).
bOligonucleotides Ce OAR1-1 refer to F09E10.3 and Ce OAR1-2 to D1054.8.