Fig. 4.

DKK-1 reduces cell-to-matrix adhesion. The cells were seeded on wells coated with collagen type I or fibronectin. The attached cells were stained with crystal violet and the optical density was measured at 630 nm (A) and the cells were photographed under a microscope (B). Values represent the means±standard error of 5 determinations per experiment from 3 independent experiments (^*p<0.05 compared to the control). (C) Immunoblot analysis of β1-integrin, a transmembrane receptor that binds to fibronectin and collagen. Probing with anti-actin antibody confirmed equal loading and transfer.