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Comparison of kinetic parameters between our trypsin sensors and commercial trypsin kits
| Trypsin sensors or kits | kcat(s−1) | Km(µM) | kcat/Km (M−1s−1) |
|---|---|---|---|
| EGFP-T1 | 0.26 ± 0.01 | 3.99 ± 0.38 | (6.52 ± 0.50) × 104 |
| EGFP-T1nb | 0.85 ± 0.06 | 4.21 ± 0.71 | (2.01 ± 0.21) × 105 |
| Bz-DL-Arg-pNA | 1.33 ± 0.17 | (3.00 ± 0.50) × 103 | (4.44 ± 0.41) × 102 |
| Bz-DL-Arg-AMC | 0.25 ± 0.01 | 77.44 ± 8.46 | (3.22 ± 0.21) × 103 |
| Boc-Gln-Ala-Arg-AMC | 1.41 ± 0.05 | 9.27 ± 0.95 | (1.52 ± 0.12) × 105 |
